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Liofilchem gradient diffusion strips
Gradient Diffusion Strips, supplied by Liofilchem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient+diffusion+strips/diffusion+gradient+strips/pmc13016996-66-9-12
Average 86 stars, based on 1 article reviews
gradient diffusion strips - by Bioz Stars, 2026-10
86/100 stars

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Diffusion-based Assay:

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: Ampicillin sodium salt, cefotaxime sodium salt, and ceftriaxone disodium salt hemi(heptahydrate) were purchased from Sigma-Aldrich. .. Gradient diffusion strips (Liofilchem MIC Test Strips) on Mueller–Hinton fastidious agar (BD) were routinely used for all isolated single clones evolved in the evolution experiment to determine the MICs of ampicillin, cefotaxime, and ceftriaxone. .. To check for collateral effects, 17 clones (3–4 per evolved population, selected based on differing mutational patterns and/or distinct beta-lactam MICs) were additionally tested for clarithromycin, kanamycin, colistin, vancomycin, levofloxacin, rifampicin, amikacin, tetracycline, gentamicin, and meropenem susceptibility using Liofilchem MIC Test Strips.

Article Title: Clinical Features and Outcomes of Invasive Fusarium Infections from a Singaporean Centre: Is It Time to Redefine How We Assess Treatment Outcomes?
Article Snippet: Voriconazole, posaconazole and amphotericin B MICs were carried out using gradient diffusion strips (bioMérieux; Marcy-l’Etoile, France) from 2011 and Sensititre Yeast One assay (YO10) by Trek Diagnostics Systems (ThermoFisher Scientific; West Sussex, United Kingdom) from 2016 onwards. .. Isavuconazole was performed using gradient diffusion strips (Liofilchem; Roseto degli Abruzzi, Italy). ..

Article Title: Plasmid-mediated resistance in Neisseria gonorrhoeae clinical strains from South Poland, 2014-2021.
Article Snippet: of gonococcal strains to antibiotics and the prevalence of both plasmid-mediated and chromosomal resistance, varies by geographic region and changes over time (Tapsall et al. 2009; Town et al. 2020).. Since the 1930s, when sulfonamides and later penicillin were introduced for the treatment of gonorrhoea, N. gonorrhoeae has gradually developed resistance to successive classes of antibiotics, including sulfonamides, penicillins, tetracyclines, fluoroquinolones, macrolides, and cephalosporins (Grad et al. 2016; Ohnishi et al. 2011; Unemo and Shafer 2014).. Due to this fact the World Health Organization (WHO) has included this pathogen on the list of bacteria for which the development of new antimicrobial drugs is urgently needed (WHO 2017).

Article Title: Multicentric evaluation of the MTS-SAS® for reliable antibiotic synergy testing in clinical laboratories.
Article Snippet: .. For the multicenter evaluation, each of the 11 participating laboratories tested MICs of ciprofloxacin, meropenem, colistin, ceftazidime, amikacin, and rifampicin using commercial gradient diffusion strips (MIC Test Strip, Liofilchem, Italy). ..

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: .. Isolated clones were grown in 3 mL of HTM to determine MICs of ampicillin, cefotaxime, and ceftriaxone using gradient diffusion strips (Liofilchem MIC Test Strips) and to isolate DNA using the DNeasy UltraClean Microbial Kit (Qiagen) for DNA sequencing. .. Short-read DNA libraries were prepared from extracted genomic DNA with a modified Illumina Nextera XT library kit protocol as described previously ( ).

Article Title: Novel KPC-2 plasmid in a clinical Salmonella Rissen selected by antibiotic pressure.
Article Snippet: Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Seville, Spain; Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain; Department of Medicine, Faculty of Medicine, University of Seville, Seville, Spain; Department of Microbiology, Faculty of Medicine, University of Seville, Seville, Spain.

Article Title: Impact of loading dose β-lactam therapy on outcomes of KPC-producing Klebsiella pneumoniae bloodstream infections in non-ICU patients: a real world study.
Article Snippet: .. MIC values for ceftazidime–avibactam and meropenem–vaborbactam were determined using gradient diffusion strips (Liofilchem, Italy), with discordant or high-end values validated by broth microdilution. ..

Article Title: Impact of loading dose β-lactam therapy on outcomes of KPC-producing Klebsiella pneumoniae bloodstream infections in non-ICU patients: a real world study
Article Snippet: .. MIC values for ceftazidime–avibactam and meropenem–vaborbactam were determined using gradient diffusion strips (Liofilchem, Italy), with discordant or high-end values validated by broth microdilution. ..

Isolation:

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: Ampicillin sodium salt, cefotaxime sodium salt, and ceftriaxone disodium salt hemi(heptahydrate) were purchased from Sigma-Aldrich. .. Gradient diffusion strips (Liofilchem MIC Test Strips) on Mueller–Hinton fastidious agar (BD) were routinely used for all isolated single clones evolved in the evolution experiment to determine the MICs of ampicillin, cefotaxime, and ceftriaxone. .. To check for collateral effects, 17 clones (3–4 per evolved population, selected based on differing mutational patterns and/or distinct beta-lactam MICs) were additionally tested for clarithromycin, kanamycin, colistin, vancomycin, levofloxacin, rifampicin, amikacin, tetracycline, gentamicin, and meropenem susceptibility using Liofilchem MIC Test Strips.

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: .. Isolated clones were grown in 3 mL of HTM to determine MICs of ampicillin, cefotaxime, and ceftriaxone using gradient diffusion strips (Liofilchem MIC Test Strips) and to isolate DNA using the DNeasy UltraClean Microbial Kit (Qiagen) for DNA sequencing. .. Short-read DNA libraries were prepared from extracted genomic DNA with a modified Illumina Nextera XT library kit protocol as described previously ( ).

Clone Assay:

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: Ampicillin sodium salt, cefotaxime sodium salt, and ceftriaxone disodium salt hemi(heptahydrate) were purchased from Sigma-Aldrich. .. Gradient diffusion strips (Liofilchem MIC Test Strips) on Mueller–Hinton fastidious agar (BD) were routinely used for all isolated single clones evolved in the evolution experiment to determine the MICs of ampicillin, cefotaxime, and ceftriaxone. .. To check for collateral effects, 17 clones (3–4 per evolved population, selected based on differing mutational patterns and/or distinct beta-lactam MICs) were additionally tested for clarithromycin, kanamycin, colistin, vancomycin, levofloxacin, rifampicin, amikacin, tetracycline, gentamicin, and meropenem susceptibility using Liofilchem MIC Test Strips.

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: .. Isolated clones were grown in 3 mL of HTM to determine MICs of ampicillin, cefotaxime, and ceftriaxone using gradient diffusion strips (Liofilchem MIC Test Strips) and to isolate DNA using the DNeasy UltraClean Microbial Kit (Qiagen) for DNA sequencing. .. Short-read DNA libraries were prepared from extracted genomic DNA with a modified Illumina Nextera XT library kit protocol as described previously ( ).

AST Assay:

Article Title: Plasmid-mediated resistance in Neisseria gonorrhoeae clinical strains from South Poland, 2014-2021.
Article Snippet: of gonococcal strains to antibiotics and the prevalence of both plasmid-mediated and chromosomal resistance, varies by geographic region and changes over time (Tapsall et al. 2009; Town et al. 2020).. Since the 1930s, when sulfonamides and later penicillin were introduced for the treatment of gonorrhoea, N. gonorrhoeae has gradually developed resistance to successive classes of antibiotics, including sulfonamides, penicillins, tetracyclines, fluoroquinolones, macrolides, and cephalosporins (Grad et al. 2016; Ohnishi et al. 2011; Unemo and Shafer 2014).. Due to this fact the World Health Organization (WHO) has included this pathogen on the list of bacteria for which the development of new antimicrobial drugs is urgently needed (WHO 2017).

Chocolate:

Article Title: Plasmid-mediated resistance in Neisseria gonorrhoeae clinical strains from South Poland, 2014-2021.
Article Snippet: of gonococcal strains to antibiotics and the prevalence of both plasmid-mediated and chromosomal resistance, varies by geographic region and changes over time (Tapsall et al. 2009; Town et al. 2020).. Since the 1930s, when sulfonamides and later penicillin were introduced for the treatment of gonorrhoea, N. gonorrhoeae has gradually developed resistance to successive classes of antibiotics, including sulfonamides, penicillins, tetracyclines, fluoroquinolones, macrolides, and cephalosporins (Grad et al. 2016; Ohnishi et al. 2011; Unemo and Shafer 2014).. Due to this fact the World Health Organization (WHO) has included this pathogen on the list of bacteria for which the development of new antimicrobial drugs is urgently needed (WHO 2017).

Stripping Membranes:

Article Title: Multicentric evaluation of the MTS-SAS® for reliable antibiotic synergy testing in clinical laboratories.
Article Snippet: .. For the multicenter evaluation, each of the 11 participating laboratories tested MICs of ciprofloxacin, meropenem, colistin, ceftazidime, amikacin, and rifampicin using commercial gradient diffusion strips (MIC Test Strip, Liofilchem, Italy). ..

DNA Sequencing:

Article Title: Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogen Haemophilus influenzae
Article Snippet: .. Isolated clones were grown in 3 mL of HTM to determine MICs of ampicillin, cefotaxime, and ceftriaxone using gradient diffusion strips (Liofilchem MIC Test Strips) and to isolate DNA using the DNeasy UltraClean Microbial Kit (Qiagen) for DNA sequencing. .. Short-read DNA libraries were prepared from extracted genomic DNA with a modified Illumina Nextera XT library kit protocol as described previously ( ).



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